3DNA FISH Probes

Genisphere's 3DNA Starfish technology offers researchers a more sensitive and specific alternative to conventional cDNA probes. Starfish in situ probes allow the researcher to select gene-specific oligonucleotide sequences (30-100+ nucleotides) which are attached by Genisphere onto fluorescent 3DNA dendrimer molecules, providing a simple oligo probe capable of delivering dozens to hundreds of fluorescent labels to each target molecule. The Starfish hybridization protocols are similar to those used with fluorescently labeled oligo or cDNA probes and have been optimized for cytoplasmic targets. Protocols for chromosomal targets are under development. The Starfish product line also includes fluorescently-labeled positive and negative control kits.

3DNA FISH Probes
Starfish™ Custom Probe

Catalog Number A100270 (Cy™3 label, Cy™5 label, Alexa Fluor 488 label, Alexa Fluor 546 label, Alexa Fluor 594 label, Alexa Fluor 647 label) 3DNA Starfish probes have many advantages compared to ordinary fluorescent cDNA probes:

3DNA FISH Probes
Starfish Positive/Negative Control Kit

Catalog Numbers
B100935 (Cy™3 label);
B100945 (Alexa Flour 488);
B100955 (Alexa Flour 594)

The Starfish Positive control is a fluorescent 3DNA probe with an oligo dT(24) sequence that is capable of detecting poly (A)+ sequences in messenger RNA.
The Starfish Negative control is a fluorescent 3DNA dendrimer without any target-specific sequence attached. This reagent is useful for detecting non-specific background caused by inadequate post hybridization washing conditions.

Whole Transcriptome Linear RNA Amplification

The most versatile and accurate linear whole transcriptome amplification kits available.

Genisphere's RNA Amplification product line provides sense-strand RNA for any application. Check out our product selection guide to chose the best kit for your RNA samples.
Two rounds of RNA Amplification
RampUP & Ramp UP Plus - Optimized to amplify RNA by >250,000 with 2 rounds. The use of Genisphere's proprietary Tandem RNA Polymerase Promoter (TRPP) technology allows highly accurate, reproducible, high throughput RNA amplification. Input Total RNA: up to 10ng. Downstream compatibility with with qRT-PCR, glass microarrays, and GeneChip arrays.
One round of RNA Amplification
SenseAMP & SenseAMP Plus - Optimized to amplify RNA by >1000 fold with 1 round. Allows highly accurate, reproducible, high throughput RNA amplification. Input Total RNA: 25ng-2ug. Downstream compatibility with with qRT-PCR, glass microarrays, and GeneChip arrays (SenseAMP Plus).
Low Molecular Weight RNA Amplification
SenseAMP Plus - Optimized protocol for Low Molecular Weight RNA samples, including severely degraded RNA, piRNA, miRNA, snRNA, hnRNA, etc. Amplify by >1000 fold with 1 round. Allows highly accurate, reproducible, high throughput RNA amplification. Downstream compatibility with with qRT-PCR, glass microarrays and ELOSA applications.